Why Calibrate an Incubator?

Over time, incubator sensors can drift, leading to discrepancies between the displayed and actual conditions. Regular calibration helps:

• Maintain consistent environmental conditions.

• Ensure the accuracy of experimental results.

• Comply with regulatory standards.

Step-by-Step Guide to Calibrating an Incubator

1. Preparation

• Gather Necessary Tools:

• A calibrated reference thermometer (preferably with 0.1°C readability and ±0.2°C accuracy) .

• A calibrated hygrometer for humidity calibration.

• Data logger (optional) for continuous monitoring.

• Ensure a Stable Environment:

• Place the incubator in a location free from drafts and direct sunlight.

• Allow the incubator to stabilize at the desired setpoint temperature for at least 30 minutes.

2. Temperature Calibration

• Placement of Sensors:

• Position the reference thermometer at various locations inside the incubator (e.g., top, middle, bottom) to assess uniformity .

• Recording Readings:

• After stabilization, record the temperatures from both the incubator display and the reference thermometer.

• Calculate the difference between the readings.

• Adjusting the Incubator:

• If discrepancies exceed acceptable limits (typically ±0.5°C), adjust the incubator's temperature settings accordingly.

• Some incubators allow for internal calibration adjustments; refer to the manufacturer's manual for specific instructions .

3. Humidity Calibration (If Applicable)

• Placement of Hygrometer:

• Place the calibrated hygrometer inside the incubator, ensuring it's not obstructed.

• Recording Readings:

• After stabilization, compare the incubator's humidity display with the hygrometer's reading.

• Adjusting the Incubator:

• If discrepancies are noted, adjust the incubator's humidity settings as per the manufacturer's guidelines.

4. Documentation

• Record all calibration data, including:

• Date and time of calibration.

• Setpoints and actual readings.

• Adjustments made.

• Equipment used for calibration.

• Maintain a calibration log for future reference and compliance purposes.

Best Practices

• Regular Calibration:

• Perform calibration at regular intervals, typically every 6 to 12 months, or as recommended by the manufacturer.

• Use Certified Equipment:

• Ensure that all reference instruments used for calibration are themselves calibrated and certified.

• Training:

• Personnel performing calibration should be adequately trained to ensure accuracy and safety.

• Follow Manufacturer's Guidelines:

• Always refer to the incubator's user manual for specific calibration procedures and recommendations.