In various scientific research experiments, the pressure calibration pump is an important tool for obtaining accurate experimental data and ensuring the smooth progress of the experiments. In physical experiments, such as studying the changes in physical properties of substances under high-pressure environments, the pressure calibration pump can precisely generate and calibrate the required high-pressure environment, enabling experimental instruments to accurately measure parameters such as phase transitions, electrical properties, and mechanical properties of substances under different pressures, providing reliable data support for in-depth exploration of the relationship between the microscopic structure and macroscopic properties of substances. In chemical experiments, for some chemical reactions involving gas reactions and sensitive to pressure, the pressure calibration pump can be used to control the pressure inside the reaction vessel and calibrate the pressure sensors, ensuring that the reactions proceed under predetermined pressure conditions, helping to study reaction kinetics, chemical equilibrium, and other issues, improving the accuracy and repeatability of chemical experiments. In biomedical experiments, such as simulating the in vivo pressure environment in cell culture experiments, the pressure calibration pump can calibrate the pressure control system in the culture device, providing suitable pressure conditions for cell growth, studying the effects of pressure on cell physiological functions, gene expression, and other aspects, promoting the research progress in the biomedical field and helping scientific researchers achieve more innovative results in various disciplinary fields.